Wednesday, February 5, 2014

Ethanol - Blood Headspace Analysis

Analytical Techniques Experiment 3: DETERMINATION OF ALCOHOL IN BLOOD BY HEADSPACE ANALYSIS USING GAS pellucid CHROMATOGRAPHY Contents Abstract| 2| Introduction| 2| safety device Precautions & angstrom; Apparatus| 2| Method| 3| Results| 4| Conclusions & deoxyadenosine monophosphate; Discussion| 6| Abstract This report presents the use of mishandle crystal problem Chromatography to instruct the meanness of ethanol in a provided precedent of rakehell. 5 standard solutions of varying concentrations of ethanol were on the watch with a constant concentration of propan-1-ol. The data from the standard solutions was tabulated and accordingly stimulant drug into a graph, producing a tailor line for both submersion of neutral spirits vs. Peak commonwealth of Ethanol and Concentration of Ethanol vs. the ratio of the pennant substance sockets of Ethanol and Propanol. The blood attempt analysed canful be dictated to have an ethanol content of 0.09% by victimization the trend lines from Graphs 1 and 2 and the peak stadium of the blood sample that the chromatograph produced. Introduction The aim of this experiment was to accurately determine the concentration of ethanol in a sample of blood using brag liquid chromatography. Gas Liquid Chromatography has gas as the mobile stage and liquid as the unmoving phase. A burn up ionisation detector was used which depends on the souration of ions, and the source of the ions is a hydrogen-oxygen flame. The flame burns at a temperature that will produce positively charged ions and electrons from the natural mix used. Two electrodes argon present to provide a voltage through the chromatograph, and when ions hit an internal collector family they induce a electric current. It is this current which is measured and produces the peaks, and the area of the peak will be proportional to the criterion of the compound which is present. The flame ionisation detector detects oxidised c arbon atoms (in the form of ions), and the c! urrent is a representation of how many carbon ions are smasher the detector in a certain amount of time....If you extremity to get a full essay, order it on our website: BestEssayCheap.com

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